Light Microscopy Resources

1. Zeiss LSM 980 with Airyscan 2 NLO Two-Photon Laser Scanning Confocal Microscope System

(Life Sciences building)

LSM 980 LSM 980
LSM 980 LSM 980
  • Inverted Axio Observer 7 with definite focus.3 motorized condenser for AI based automated sample finder and motorized XY stage.
  • Airyscan 2 multiplex high speed, joint deconvolution, super-resolution (90nm).
  • 2 MA-PMT+1 GaAsP detectors for regular confocal or 2P lasers, 1 transmitted light T-PMT for widefield imaging.
  • Laser lines: 405nm, 445nm, 488nm, 561nm, 639nm, MaiTai HP tunable 2P laser: 680nm – 1040nm.
  • Objectives: 10X, 20X, 40X, 63X, 100X.
  • Equipped with a dark heated incubator chamber for live imaging.
  • Equipped with a LSM Inverter for upright application with 2P system.
  • Capable of FRAP, FLIP, FRET, 3D, spectral unmixing, colocalization, ROI measurement, line and spot scan, multiposition assay, time lapse, photo-activation, live cell imaging, and small live animal imaging.    

2. Nikon N-SIM Super Resolution Microscope System

(Life Sciences building)

N-SIM

  • N-SIM (Structured Illumination Microscopy): resolution 100nm, 2x higher resolution than conventional microscopy.
  • Inverted Nikon Eclipse Ti research microscope with a motorized stage.
  • 100x objective only for N-SIM imaging.
  • Laser lines: 405nm, 488nm, 561nm, 640nm.
  • 3D capable. 

3. Nikon N-STORM Super Resolution Microscope System

(Life Sciences building)

N-STORM

  • N-STORM (Stochastic Optical Reconstruction Microscopy): resolution 20nm, 10x higher resolution than conventional microscopy.
  • Inverted Nikon Eclipse Ti research microscope with a motorized stage.
  • Laser lines: 405nm, 488nm, 561nm, 647nm.
  • 3D capable, TIRF capable. 

STORM Protocol-Sample Preparation

4. Leica TCS SP8X Laser Scanning Confocal Microscope System

(AERTC building, R & D Park)

TCS SP8X

  • Upright Leica DM 6000 microscope with a motorized XY-Stage.
  • Laser lines: White Light Laser (WLL) 470-670 nm), UV 405 nm. Detection range: 400-800 nm.
  • Internal detection channels: 2xPMT, 2x HyD.
  • Equipped with Tokai Hit Stage Incubator providing 37˚ C and 5% CO2 for live cell imaging.
  • Tandem scanner 8 KHz with non-resonant mode and resonant mode.

5. Zeiss LSM 510 META Laser Scanning Confocal Microscope System

(Life Sciences building)

LSM 510

  • Inverted Zeiss Axiovert 200M microscope with a manual control stage.
  • Laser lines: 458nm, 477nm, 488nm, 514nm, 543nm, 633nm.
  • Equipped with incubator chamber and a Zeiss CTI-Controller 3700 for CO2 and temperature control for live cell imaging.
  • Capable of FRAP, FLIP, FRET, 3D, time lapse, photo-activation, and spectral imaging.

6. Image Processing Software

(Life Sciences building)

Image Processing Software

  • One standalone workstation with Nikon NIS-Element (Ar) software, STORM images, and SIM images off-line analysis licenses.
  • One standalone workstation with Zeiss LSM software (version 4.0) off-line image analysis license